Citrus huanglongbing: validation of Real-Time PCR (qPCR) for the detection of Candidatus Liberibacter asiaticus and Candidatus Liberibacter americanus in Colombia
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چکیده
منابع مشابه
Quantitative real-time PCR for detection and identification of Candidatus Liberibacter species associated with citrus huanglongbing.
Citrus huanglongbing (HLB, ex greening) is one of the most serious diseases of citrus. Different forms of the disease are caused by different Candidatus Liberobacter species, Candidatus Liberibacter asiaticus (Las), Ca. L. africanus (Laf) and Ca. L. americanus (Lam). The pathogen is transmitted by psyllid insects and by budding with contaminated plant materials. The vector psyllid Diaphorina ci...
متن کاملQuantitative distribution of 'Candidatus Liberibacter asiaticus' in citrus plants with citrus huanglongbing.
Citrus huanglongbing (HLB), or greening disease, is strongly associated with any of three nonculturable gram-negative bacteria belonging to 'Candidatus Liberibacter spp.' 'Ca. Liberibacter spp.' are transmitted by citrus psyllids to all commercial cultivars of citrus. The diseases can be lethal to citrus and have recently become widespread in both São Paulo, Brazil, and Florida, United States, ...
متن کاملCultivation of 'Candidatus Liberibacter asiaticus', 'Ca. L. africanus', and 'Ca. L. americanus' associated with huanglongbing.
A new medium designated Liber A has been designed and used to successfully cultivate all three 'Candidatus Liberibacter spp.,' the suspect causative agents of huanglongbing (HLB) in citrus. The medium containing citrus vein extract and a growth factor sustained growth of 'Ca. Liberibacter spp.' for four or five single-colony transfers before viability declined. Colonies, positive for 'Ca. L. as...
متن کاملInfection of Wampee and Lemon by the Citrus Huanglongbing Pathogen ( Candidatus Liberibacter Asiaticus ) in China
Single-step and nested polymerase chain reactions (PCR) were used to determine the presence of Candidatus Liberibacter asiaticus, the phloem-limited bacterial pathogen of Huanglongbing (HLB), in leaves of wampee [Clausena lansium (Lour.) Skeels], lemon [Citrus limon (L.) Burm.] and several other citrus species. Specific PCR products were obtained when single-step PCR and nested PCR were used to...
متن کاملConvenient Detection of the Citrus Greening (Huanglongbing) Bacterium ‘Candidatus Liberibacter asiaticus’ by Direct PCR from the Midrib Extract
A phloem-limited bacterium, 'Candidatus Liberibacter asiaticus' (Las) is a major pathogen of citrus greening (huanglongbing), one of the most destructive citrus diseases worldwide. The rapid identification and culling of infected trees and budwoods in quarantine are the most important control measures. DNA amplification including conventional polymerase chain reaction (PCR) has commonly been us...
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ژورنال
عنوان ژورنال: Agronomía Colombiana
سال: 2014
ISSN: 2357-3732,0120-9965
DOI: 10.15446/agron.colomb.v32n3.44069